Common problems of cryopreservation

Date:2022-06-29 17:55:59 Wednesday

Summary:Common problems of cryopreservation:Q: can the cryopreservation tube be sterilized?Answer: Although the cryopreservation tube is made of polyethylene material, we do not recommend sterilization.Q: what is the difference between the internal and exter......

Common problems of cryopreservation:
Q: can the cryopreservation tube be sterilized?
Answer: Although the cryopreservation tube is made of polyethylene material, we do not recommend sterilization.

Q: what is the difference between the internal and external screw caps of the cryopreservation tube?
Answer: the internal rotating cryopreservation tube is designed for samples frozen in the gas phase, liquid phase and nitrogen phase, and the silicone pad at the pipe orifice enhances the sealing of the cryopreservation tube; The external rotating cryopreservation tube is designed for freezing samples in mechanical equipment such as refrigerator. The threaded cover of the external rotating cover can reduce the pollution probability when handling samples.
Q: heat sealing is required when using sealed tubes. Will this affect cell activity?
Answer: before adding the sample to the cryopreservation tube, the cryopreservation tube should be placed in a 4 ℃ ice bath. When the tube is sealed by heat, the temperature rise in the cryopreservation tube is not enough to destroy the cell activity. It is necessary to add cryoprotectant at this stage.
Q: can the cryopreservation tube be immersed in liquid nitrogen (liquid phase) to freeze the sample?
Answer: the internal rotating cryopreservation tube can be stored in liquid nitrogen, and the sealing property is enhanced by the silica gel gasket. However, at ultra-low temperature, any sealing material becomes extremely fragile. Theoretically, as long as it is a mechanical seal, there is a risk of leakage. In order to prevent the risk of leaking products, the mechanical seal cryopreservation tube must be sealed in a protective bag.