Cryopreservation of Adipose-Derived Stem Cells
Date:2022-12-01 15:24:33 Thursday
Summary:Various techniques are used to freeze cells. Some methods are commercially available, while others are user-developed systems. The cell freezing tube is one type of tube. It is made of polypropylene and is leak-proof. It provides convenience for the ......
Various techniques are used to freeze cells. Some methods are commercially available, while others are user-developed systems. The cell freezing tube is one type of tube. It is made of polypropylene and is leak-proof. It provides convenience for the operation and also reduces pollution.
The cell freezing tube is made of polypropylene, a transparent polymer. It was specially manufactured to guarantee leak-free and stable quality. It has a screwing section and an extension section. Both sections of the tube body are provided with female thread structure. The tube cover is also provided with an extending section.
The pipe lid is provided with a female thread structure, and an internal thread structure is arranged on the inner wall of the screwing section. The pipe lid is provided with an extension and supporting and bracketing structure. The supporting and bracketing structure is arranged on the outer wall of the pipe lid.
The cryopreservation process of the present invention can be carried out by using an ultra-low-temperature freezer or a nitrogen tank. This method is advantageous for lymphocyte stability and proliferation. It is also a good method for the long-term preservation of cells.
The present invention relates to adipose-derived stem cells. A serum-free freezing medium is used for long-term preservation of adipose-derived stem cell. This medium is superior to the serum-containing freezing medium. This medium can improve the viability of cells, improve cell adherence, and increase the differentiation capacity of adipose-derived cells.
Adipose-derived stem cells are well preserved, and their survival percentage is high. Cryopreservation in a serum-free medium can solve the defects of unstable freezing quality and adherence capacity of adipose-derived cell.