Methods of microbial inoculation

Date:2022-02-09 14:40:55 Wednesday

Summary:1. Streaking inoculation: separate the various bacterial species mixed together in the culture, and allow the bacterial species to grow dispersedly to form a single colony. Common methods include segmented streaking and continuous streaking. 2. Thre......

1. Streaking inoculation: separate the various bacterial species mixed together in the culture, and allow the bacterial species to grow dispersedly to form a single colony. Common methods include segmented streaking and continuous streaking.

2. Three-point inoculation: Move the bacteria in the culture to the flat surface and form an equilateral triangle, so that the bacteria on the three points form colonies respectively, which is convenient for observation, and is often used to study the morphology of molds.

3. Puncture inoculation: Dip the bacteria with an inoculation needle, and puncture it in a straight line along the center of the semi-solid medium to the bottom of the tube, which is often used for the preservation of anaerobic bacteria.

4. Pour and mix inoculation: put the microorganisms in a petri dish, pour it into a solid medium with a temperature of 45°C and shake it up. After solidification, put it in a suitable environment for cultivation.

5. Coating and inoculation: pour the plate, wait until the plate solidifies, and wait for the coating rod to spread and roll on the surface of the plate, so that the bacterial liquid is evenly distributed on the plate.

6. Liquid inoculation: Pour the strains in the solid medium into the liquid medium, or use a pipette to move the strains from the liquid medium to another liquid medium.

7. Inoculation by injection: The microorganisms are transferred into the living body by injection, and vaccination is common.

8. In vivo inoculation: The virus is transplanted into a living body or cultured in a living tissue, because the virus can only survive in the living body.