Pipette Knowledge Shar

Date:2022-05-19 0:00:00 Thursday

Summary:Quite a lot of people think that the Serological pipette is a very simple instrument, its operation is also very simple, there is no need for words and no training. In fact? Good pipette usage habits are of great benefit to extending the service lif......

Quite a lot of people think that the Serological pipette is a very simple instrument, its operation is also very simple, there is no need for words and no training. In fact? Good pipette usage habits are of great benefit to extending the service life of the pipette, improving the precision of pipetting and maintaining the health of the user. The following is a list of some common bad operating behaviors in the laboratory, and see if you have the same habitual mistakes?

Pre-wash tips

After we install a new pipette tip or increase the capacity value, the liquid that needs to be transferred should be sucked and discharged two to three times. This is to form a homogeneous liquid film on the inner wall of the pipette tip to ensure the accuracy of pipetting and Accuracy makes the entire pipetting process extremely reproducible.

Secondly, when absorbing organic solvents or highly volatile liquids, volatile gases will form negative pressure in the white sleeve chamber, resulting in liquid leakage. At this time, we need to pre-wash four to six times to let the gas in the white sleeve chamber. When saturation is reached, the negative pressure will disappear automatically.

For viscous liquids, the pipette tip can be pre-wetted to achieve precise pipetting. First inhale the sample liquid, and pump it out. The inner wall of the pipette tip will absorb a layer of liquid to saturate the surface adsorption, then inhale the sample liquid, and finally dispense the liquid. The volume will be very precise.

Aspiration speed.

Have you ever had fluid suck back into the piston while you were operating?

It is often seen in the laboratory that in order to "improve efficiency", the user quickly releases the thumb when aspirating liquid, which causes the liquid to quickly flush into the tip.

As everyone knows, this forces the liquid to form a turbulent state after entering the tip, so that for small and medium-range pipettes, invisible vapor will be generated into the inside of the pipette, and for large-scale pipettes You will see the liquid flush directly to the inside of the pipette. The end result is to contaminate or even corrode the piston of the pipette, which is easy to cause cross-contamination of the sample, which also affects the accuracy.