Precautions and common problems of aseptic operation

Date:2022-01-22 9:34:15 Saturday

Summary:Doing different experiments, there will be small differences in operation, and it is impossible to describe every action, so you must pay attention to your actions when experimenting. The general concern is to minimize the possibility of contaminatio......

Doing different experiments, there will be small differences in operation, and it is impossible to describe every action, so you must pay attention to your actions when experimenting. The general concern is to minimize the possibility of contamination with each operation, so the movements should be minimized.

Distance, try to place the items you need as close to your hand as possible. The closer you are, the less you move.

Exposure, the longer an item is moved in the air, the greater the probability of exposure to airborne particles. The longer the reagent bottle is open in the air, the more airborne particles that fall into it. Burning the bottle neck and pipette to fix the particles on the surface and creating an upward airflow can reduce the number of dropped particles.

Burning is not for killing germs, just burn on the flame for 1 to 3 seconds.

Movement, movement can cause the flow of air. The faster you move, the faster the flow, so only do those necessary movements slowly, without waving the pipette in the air or crossing your hands in the work area.
Raining Tip

Infusion, infusion will produce aerosols, air and some unknown pollutants will also be mixed, and the liquid remaining in the bottle mouth becomes an important source of pollution because it can contact the outside world. Whenever possible, use only pipettes when transferring liquids.

The pipette can be used to transfer up to 50ml of liquid. Make sure that the pipette is fixed on the pipetting aid when using it. The 50ml pipette is relatively large. trickle down.

Open the lid while keeping the reagent bottle at an approximately 45° angle to the table top. This minimizes air contamination and the generation of minimal aerosols during pipetting.

If the cover must be removed, place it upside down on a clean countertop.

There are two ways to put the cap of the reagent bottle, positive and reverse, both of which are feasible, just choose one. If the lid is upside down, make sure your hands don't pass over it, and make sure the table top is clean when facing up.