There is also a big problem with the angle of the pipette when picking up

Date:2022-10-14 11:07:09 Friday

Summary:In laboratories where pipettes are frequently used, the sound of tapping the tip can often be heard one after another, which adds a little music to the boring laboratory life. After a closer look, it turns out that the user is afraid that the tip can......

In laboratories where pipettes are frequently used, the sound of tapping the tip can often be heard one after another, which adds a little music to the boring laboratory life. After a closer look, it turns out that the user is afraid that the tip cannot be installed tightly. After the tip is installed on the pipette, he taps several times in the tip box, hoping to ensure the sealing of the pipette through this impact force. sex. So what's wrong with this operation?

First, under the action of impact, the tip may be deformed and affect the pipetting accuracy;

Second, the part of the pipette handle that is in contact with the tip will gradually become rough and difficult to ensure tightness after multiple strong frictions. In order to achieve good tightness, greater impact force is required, and the vicious cycle begins. ;

Third, the handle of the small-scale pipette is relatively thin, and it is likely to become a fishing hook under the constant impact and destruction;

Fourth, sometimes the impact force is greater than the force the pipette can withstand when it falls on the ground, and some precision components in the pipette may be overwhelmed and strike at any time.
When pipetting samples that are close to or reach the maximum volume of the tip, the large angle of the pipette can easily allow the liquid to slip into the handle of the pipette, thereby contaminating or even corroding the piston of the pipette, and it is also easy to cause sample damage. cross-contamination;

The large-angle inclination of the pipette also increases the contact surface between the outer wall of the tip and the liquid, so that when the tip leaves the liquid surface, more liquid remains on the outer wall, and it is easy to accompany the liquid in the tip when discharging. Leaving the tip, thereby reducing the precision of pipetting, and these residual liquids are likely to be fatal for small volume (such as 2ul and 10ul) pipettes.