Why does the culture surface of cell culture consumables need TC treatment? Cell culture knowledge sharing~

Date:2022-08-05 0:00:00 Friday

Summary: AMNGENT CONSUMABLES 01 What is Cell Culture? Cell culture refers to a method of mimicking the in vivo environment (sterility, suitable temperature, pH, certain nutritional conditions, etc.) in vitro to survive, ......

AMNGENT CONSUMABLES

What is Cell Culture?

Cell culture refers to a method of mimicking the in vivo environment (sterility, suitable temperature, pH, certain nutritional conditions, etc.) in vitro to survive, grow, reproduce, and maintain its main structure and function, which is the extraction of cells from animals or plants and then cultured in an artificial environment for scientific research.

A large number of cells can be obtained through cell culture, and cell signal transduction, cell anabolism, cell growth and proliferation can be studied. At the same time, cell culture technology is also a commonly used research method in life sciences, which can exclude the influence of neurohumoral factors and the interference of liver and kidney detoxification functions, and observe the direct effect of certain factors or drugs on cell culture. However, the cultured cells lose the restriction of cells in the body and the overall regulatory effect, and the cell morphology and function will change to a certain extent. Culture methods and experimental reagents have a certain influence on cell morphology and function, such as trypsin can destroy cell surface receptors, enzymes, antigens, etc. In addition, long-term in vitro culture cells may undergo aneuploidy changes due to factors such as repeated passage, cryopreservation and manipulation, and they are characterized by immortality or cancer.

Suspension cell culture

There are two basic methods of cell culture: suspension cell culture and adherent cell culture.

Suspension cells refer to cell growth that does not depend on the surface of support and grows in suspension in culture medium, such as lymphocytes, hematopoietic cell lines, etc. This culture method is easier to pass, and the process does not require enzymatic digestion or mechanical dissociation. However, daily cell counts and viability assays are required to observe their growth. Growth can be stimulated by diluting the culture while agitation (i.e., shaking or stirring) is required for adequate gas exchange.

This method can be used for mass protein production, bulk cell collection, and many research applications.

Adherent cell culture

Adherent cells are divided into dermal cell type, fibroblast type, migratory cell type, and polytype cell type.

Adherent culture refers to the growth of cells in a monolayer on an artificial substrate. When viewed under the microscope, adherent cells are stretched at the bottom of the bottle and extended into spindle or irregular triangles or sectors or other forms, and the cells do not move when the culture medium is shaken.

Cultured cells generally resemble spheroids before they are attached to the substrate, and when attached to the substrate, the cells will gradually stretch to form a certain shape, such as fibroblasts or epithelial cells.

This culture method is suitable for most cell types, such as in animal cell culture, where there must be a surface of support that can be attached to which it relies on its own secretion or adhesion factors in the medium to grow and proliferate in vitro animal cultured cells.

The growth of adherent culture cells is limited by surface area, which may limit production yield, while adherent culture requires TC-treated cell culture vessels on the surface.

Why do you need TC treatment?

The full name of TC is Tissue culturetreated, TC treatment means that the vessel has been modified on the surface, which is suitable for the culture of adherent cells. Cells grown in suspension do not necessarily need such specially treated vessels. However, cell culture dishes that have been modified on the surface are generally suitable for culture of suspension cells.

Most animal cells need to be attached to a solid-phase interface to grow. Only at a hydrophilic solid-phase interface can cells attach.

The earliest cell culture vessels were made of glass. Since the surface of the glass is hydrophilic, ordinary cells can be attached to grow without special treatment. Polystyrene has good light transmittance, good strength and plasticity, and no toxicity, becoming the preferred material for disposable cell culture consumables such as disposable cell culture dishes and cell culture plates, but the surface of polystyrene is hydrophobic, so it needs to be modified on the surface to enhance the ability of cell adsorption to bind to proteins, and become hydrophilic before it can be suitable for cell culture.

The culture surface of Amngent cell culture series consumables is treated with vacuum plasma, which uses thin gas under vacuum to collide under the action of electric field to form highly active plasma, thereby inducing the chemical bond breakage of plastic surface molecules and degradation, increasing surface roughness and increasing their hydrophilicity, so the product cell adhesion is excellent.

In addition, Amngent cell culture dishes, plates and bottles are made of highly transparent polystyrene, electron beam sterilization, no heat source, no endotoxin, stacking design, easy to stack and not easy to slip.